Hydrolysis of phosphatidylcholine in phosphatidylcholine-cholate mixtures by porcine pancreatic phospholipase A2.
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چکیده
منابع مشابه
Phospholipase C - mediated Hydrolysis of Phosphatidylcholine
We have investigated the effect of cis-diamminedichloroplatinum(II) (CDDP) on signal transduction pathways. CDDP treatment did not cause any change in the binding of 1H1phorbol dibutyrate to PC-9 (human lung adenocarcinoma cell line) cells, a measure of protein kinase C activation. However, 2-h CDDP treatment (20 gg/ml) caused 200% increase in 1,2-sn-diacylglycerol (DAG) production and 50% decr...
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29. Brooks, G., Evans, A. T., Aitken, A. & Evans, F. J. (1987) Cancer Lett. 38,165-170 Evans, A. T., Brooks, G. & Evans, F. J. (1990) Cancer Lett. 49,2529 Evans, F. J., Kinghorn, A. D. & Schmidt, R. J. (1975) Acta Pharmacol. Toxicol. 37,250-256 Tapley, P. M. & Murray, A. W. (1985) Eur. J. Biochem. 151,419-423 Edwards, M. C., Williamson, E. M., Formakong, E., Brooks, G. & Evans, F. J. (1987) Bio...
متن کاملPhosphatidylcholine hydrolysis is required for pancreatic cholesterol esterase- and phospholipase A2-facilitated cholesterol uptake into intestinal Caco-2 cells.
Pancreatic secretion is required for efficient cholesterol absorption by the intestine, but the factors responsible for this effect have not been clearly defined. To identify factors involved and to investigate their role in cholesterol uptake, we studied the effect of Viokase(R), a porcine pancreatic extract, on cholesterol uptake into human intestinal Caco-2 cells. Viokase is capable of facil...
متن کاملHydrolysis of dipalmitoylphosphatidylcholine small unilamellar vesicles by porcine pancreatic phospholipase A2.
The hydrolysis of small unilamellar vesicles made of dipalmitoylphosphatidylcoline by pancreatic phospholipase A2 has been studied under various conditions of temperature and enzyme and substrate concentration using the following three different experimental protocols. When the enzyme was added to the substrate vesicles after being separately adjusted to the temperature of the experiments hydro...
متن کاملPhospholipase C-mediated hydrolysis of phosphatidylcholine is activated by muscarinic agonists.
The phospholipase C-catalysed breakdown of inositol-containing phospholipids is an important source of diacylglycerol in cells stimulated by several agonists. However, recent experimental evidence suggests that major phospholipids such as phosphatidylcholine may also be substrates of the phosphodiesteratic hydrolysis activated by hormones, growth factors and oncogene products. We show here that...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1988
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)37857-8